oSLO Platform
OSLO SELECTIVELY RESTORES THE HEALTHY CENTRAL NERVOUS SYSTEM MICROENVIRONMENT
Streptolysin O is a Cholesterol Dependent Cytolysin (CDC) and an exotoxin of group a B-hemolytic streptococcus bacteria. It has an elongated structure composed of four different domains which can create pore complexes within the cell membrane and cause hemolysis1
The oxidized form of Streptolysin O (oSLO) is non-toxic and can no longer bind with the cholesterol and form pores. It is 1/10,000th of the lethal dose concentration of reduced streptolysin.
oSLO is a biologic comprised of a low concentration of oxidized streptolysin O molecules diluted in phosphate-buffered saline.
Oxidized concentrations can affect host immune signaling 2-4
oSLO Regulates Gene Expression in Mature Human Dendritic Cells
WITHIN 48 HOURS OF EXPOSURE TO DRUG, STATISTICALLY SIGNIFICANT CAHNGES IN GENE EXPRESSION OCCUR
Background & Rationale
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Dendritic Cells (DC) are the pre-eminent sentinel antigen-presenting cells of the immune system1
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In TBI mouse model, DCs can change their subtype in the brain and throughout the circulatory and lymphatic systems after a TBI event2
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TBI at both early and late stages alters DC differentiation concomitant with a reduction of reactive oxygen species levels (ROS) in neuroprogenitor cells.
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DCs may regulate the chronic manifestations of TBI because they act on many other cell types.
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Method
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Human mature dendritic cells harvested and treated with purified oSLO.
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Cells harvested and RNA isolated – RNA concentration was determined & normalized to 20µg/mL.
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Gene microarray analysis using NanoString nCounter® Human Neuroinflammatory panel
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770 Genes & 23 Pathways
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Results
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Biochemical pathways involved include: Cytokine signaling, Integrated Stress Response, Autophagy & Growth factor signaling
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Cognitive genes downregulated:
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PLA2G4A – associated with delirium, dementia, amnesia and cognitive disorders
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CD209 – associated with psychogenic disorders
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Multiple mechanisms of action
OSLO SHOWN TO IMPACT MULTIPLE PATHWAYS TOWARD HEALING
Keratinocytes
Wound Healing
10 genes upregulated
(p< 0.031)
Macrophages
Modulate Immune Response
1 gene downregulated
3 upregulated
(p<0.037)
Neural Progenitor Cells
Brain Repair & Regeneration
