NanoSLO Platform
NANOSLO POTENTIATES FURTHER HEALING IN THE BRAIN
Streptolysin O is a Cholesterol Dependent Cytolysin (CDC) and an exotoxin of group B-hemolytic streptococcus bacteria. It has an elongated structure composed of four different domains.
Our proprietary version of Streptolysin O, which we call NanoSLO, is a recombinant protein in a form different from the classically described Streptolysin O and dosed at nanodose levels.
NanoSLO acts to promote immune system signaling in the body that allows for more complete healing FOOTNOTES?.
NanoSLO Regulates Gene Expression in Mature Human Dendritic Cells
WITHIN 48 HOURS OF EXPOSURE TO DRUG, STATISTICALLY SIGNIFICANT CAHNGES IN GENE EXPRESSION OCCUR
Background & Rationale
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Dendritic Cells (DC) are the pre-eminent sentinel antigen-presenting cells of the immune system1
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In TBI mouse model, DCs can change their subtype in the brain and throughout the circulatory and lymphatic systems after a TBI event2
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TBI at both early and late stages alters DC differentiation concomitant with a reduction of reactive oxygen species levels (ROS) in neuroprogenitor cells.
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DCs may regulate the chronic manifestations of TBI because they act on many other cell types.
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Method
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Human mature dendritic cells harvested and treated with purified oSLO.
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Cells harvested and RNA isolated – RNA concentration was determined & normalized to 20µg/mL.
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Gene microarray analysis using NanoString nCounter® Human Neuroinflammatory panel
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770 Genes & 23 Pathways
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Results
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Biochemical pathways involved include: Cytokine signaling, Integrated Stress Response, Autophagy & Growth factor signaling
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Cognitive genes downregulated:
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PLA2G4A – involved in inflammatory lipid signaling and associated with cognitive impairment
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CD209 – dendritic cell receptor implicated in immune modulation
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Dynamic Response Mechanisms
NANOSLO SHOWN TO IMPACT MULTIPLE PATHWAYS TOWARD HEALING
Keratinocytes
Wound Healing
10 genes upregulated
(p< 0.031)
Macrophages
Modulate Immune Response
1 gene downregulated
3 upregulated
(p<0.037)
Neural Progenitor Cells
Brain Repair & Regeneration
