Cellular Signaling
TARGETING GENE EXPRESSION THROUGH SIGNALING
Bioelectric signals can regulate celluar processes
The Role of Biophysics
Cells communicate intercellularly and intracellularly using principles of biophysics through various mechanisms that involve physical interactions, signaling molecules, and electrical or mechanical signals.
Cells generate and respond to endogenous electric fields through the movement of ions across cell membranes. Bioelectric signals, such as transmembrane voltage gradients or electric currents, can regulate various cellular processes, including cell proliferation, migration, and differentiation, Electrical communication between cells, facilitated by gap junctions or syncytial structures, plays critical roles in coordinating the activity of excitable cells, such as neurons and cardiac muscle cells, and in synchronizing physiological responses within tissues.
Cells employ principles of biophysics to communicate with one another across short and long distances, enabling coordinated response to environmental cues, tissue remodeling, and organismal development, By sensing and responding to physical signals, cells can integrate diverse inputs and maintain tissue integrity and function.
Cellular Signaling
Biomolecules, including DNA, RNA, and proteins, exhibit mechanical resonances, where they oscillate or vibrate at specific frequencies due to their structural properties. These resonances can be influenced by factors such as molecular conformation, binding interactions, and environmental conditions.
Epigenetic mechanisms, which control gene expression without altering the underlying DNA sequence, could potentially involve resonant molecular signaling. For example, chromatin remodeling complexes may induce mechanical vibrations or oscillations in DNA or histone proteins, affecting their accessibility to transcription factors and RNA polymerase machinery.
Signaling pathways involved in gene expression regulation may exhibit resonant behavior. For instance, signaling molecules such as kinases and phosphatases could undergo conformational changes or phosphorylation cycles that resonate at specific frequencies, influencing downstream transcriptional activity and gene expression patterns.
In RNA interference mechanisms, small RNA molecules, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs), regulate gene expression by binding to target messenger RNAs (mRNAs) and modulating their stability or translation. Resonant molecular signaling could potentially affect the efficiency or specificity of RNAi pathways by influencing the interactions between small RNAs and their target mRNAs.
The mechanical properties of nucleic acids play crucial roles in gene expression processes such as transcription, splicing, and translation. Resonant molecular signaling may impact these biophysical properties, affecting the dynamics of nucleic acid folding, unwinding, and interaction with regulatory proteins and complexes.
In summary, Resonant Molecular Signaling involves the influence of mechanical vibrations, oscillations, or harmonic interactions on the dynamic processes underlying gene regulation and expression. Our research and experiments showcase how this affects the pathogenesis of disease.
Biomolecules can exhibit mechanical resonances
Pathogenesis of Disease
The pathogenesis of diseases involve complex cascades of events. By readjusting or resetting the abnormal and dysfunctional complex system (genomic and protein interactions systems), Signaling therapies restore homeostasis by reversing or eliminating one or more of the inappropriate signals and the re-establishment toward normal biological communications within and between cells.
Cellular signaling therapies interrupt pathophysiology by selective modulation of gene expression – thereby restoring normal cell signaling function
Therapeutic agent signals reach the region of disease through intracellular, intercellular, and the extracellular matrix by energy mechanisms as very rapid electromagnetic signals. Resonant Molecular Signaling pharmacology is not dose-dependent in the manner of most pharmacological treatments, where receptor agonists or antagonists reach their targets via the circulation (including lymphatic) system (humoral systems), nervous system (electrical), or local application and are pharmacologically active in a mostly linear or curvilinear dose response function.Our novel platform is based on decades of laboratory research, animal model studies and further FDA-authorized clinical trials. It has been further validated through 3 licensing agreements held by Milkhaus or Dr McMichael:License to Elan – >30 patents covering treatment of Alzheimer’s disease and providing scientific basis for Pfizer development.License to Eudaemonic Corp – RVITM for the treatment of myositis in athletic animalsLicense to Allergy Research Group, Inc – MucolyxirTM, nutraceutical for respiratory conditions